Improved receptor assay for measuring digoxin activity.
نویسندگان
چکیده
We describe a receptor assay of digoxin activity involving Na+/K+-ATPase (EC 3.6.1.37) derived from human heart tissue. The procedure requires 500 microL of serum or plasma and incorporates one purification step, with Sep-Pak C18 cartridges (Waters). The method appears to be considerably more specific for digoxin and its cardio-active metabolites than are conventional immunoassays. Cardio-inactive metabolites and several digoxin-like factors cross react only slightly. The between-day coefficient of variation for the procedure ranged from 8.10% to 1.37% for digoxin concentrations between 0.5 and 5.0 nmol/L.
منابع مشابه
Competitive solid-phase enzyme immunoassay for measuring digoxin in serum.
In this clinically useful enzyme immunoassay of digoxin in serum, we mix sample, beta-galactosidase-labeled digoxin, and anti-digoxin Fab fragments for 30 min at room temperature, then use Sepharose-bound second antibody for phase separation, and measure the unbound enzyme activity directly in the supernate of the equilibrium reaction mixture. The immunoassay buffer--phosphate-buffered isotonic...
متن کاملA unitized enzyme-labeled immunometric digoxin assay suitable for rapid testing.
An enzyme-labeled immunometric assay has been developed for measuring digoxin concentrations in serum or plasma. Unitized, compartmentalized reagents are used with an automated sample-processing instrument. The enzyme activity of the processed sample, which is directly proportional to the digoxin concentration, is measured by using a reagent strip and the Ames Seralyzer reflectance photometer. ...
متن کاملDigoxin immunoassay with cross-reactivity of digoxin metabolites proportional to their biological activity.
Our objective was to identify commercially available digoxin immunoassays whose cross-reactivity with digoxin metabolites paralleled the pharmacological activity of the metabolites. We measured the immunoreactivity of digoxigenin bis- and monodigitoxosides, digoxigenin, and dihydrodigoxin in four immunoassays and compared the immunoactivities with pharmacological activities from studies involvi...
متن کاملImproved interassay correlation of digoxin results in patients with and without renal failure by elimination of digoxin-like immunoreactive factors.
Use of immunoassays that do not detect endogenous digoxin-like immunoreactive factors (DLIF) in serum significantly improves the between-assay correlation of digoxin results for patients. We investigated five different immunoassay methods (Abbott, Clinical Assays, Corning, Du Pont, and Syva), measuring digoxin by all five assays in sera from 38 patients in renal failure and in 40 patients with ...
متن کاملBiol. Pharm. Bull. 28(2) 340—343 (2005)
the treatment of congestive heart failure and atrial fibrillation. It is important to measure digoxin in serum from digitalized patients because the therapeutic range of serum digoxin concentration is narrow. Immunoassays are routinely used for therapeutic drug monitoring (TDM) of digoxin. The antibody with cross-reactivity to metabolites of digoxin (digoxigenin bis and monodigitoxosides, digox...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 34 2 شماره
صفحات -
تاریخ انتشار 1988